I-Fmoc-Thr(tBu)-Phe-OH

I-Fmoc-Thr(tBu)-Phe-OH

Isicelo socwaningo:
I-Fmoc-Thr(tBu)-Phe-OH iyibhulokhi yokwakha ye-dipeptide evame ukusetshenziswa ku-solid-phase peptide synthesis (SPPS). Iqembu le-Fmoc (9-fluorenylmethyloxycarbonyl) livikela i-N-terminus, kuyilapho iqembu le-tBu (tert-butyl) livikela iketango laseceleni le-hydroxyl le-threonine. Le dipeptide evikelwe icwaningwa ngendima yayo ekusizeni ukunwetshwa kwe-peptide okusebenzayo, ekwehliseni ukugqama kwe-racemization, kanye nokwenza imodeli ye-motif elandelanayo ethile ekwakhekeni kwamaprotheni nezifundo zokusebenzisana.

Umsebenzi:
I-Fmoc-Thr(tBu)-Phe-OH isebenza njengesandulela sokuhlanganisa ama-peptide ahlanganisa izinsalela ze-threonine ne-phenylalanine, ezibalulekile ekwakheni amabhondi e-hydrogen nokusebenzisana kwe-hydrophobic. Iketango laseceleni le-threonine linikela nge-polarity kanye neziza ezingaba khona ze-phosphorylation, kuyilapho i-phenylalanine yengeza uhlamvu olunephunga elimnandi nokuzinza kwesakhiwo. Le nhlanganisela iwusizo ekuklameni ama-peptide okuhlolwa kwebhayoloji, izifundo ezibophezela ama-receptor, nezinhlelo zokusebenza zokutholwa kwezidakamizwa.